The enzyme-linked immunosorbent test (ELISA), is an immunoassay labeled that is widely used and is the gold standard in immunoassays. This immunological test can detect and quantify many substances including hormones, antibodies, antigens and proteins. Complexing antigens and antibodies to detect these products produces a quantifiable result.
An antibody is a protein type produced by the immune system. This protein type contains specific regions that can bind to antigens. An antigen is a protein from a foreign source that is bound to an antibody. This triggers a series of events in the body's immune systems. You can buy Elisa kits via https://www.bosterbio.com/elisa-kits.
This interaction is used in ELISA testing. It allows you to identify specific antigens and protein antibodies with very small samples. ELISA testing can be used to diagnose HIV infection, blood typing, and pregnancy tests. This article will cover the clinical significance and basic principles of the ELISA.
ELISAs can be performed on polystyrene plates. Usually, these plates are 96-well and coated with protein to bind very strongly. ELISA types vary in the requirements for testing. They require a primary or secondary detection antibody, antigen and coating antigen as well as buffer, wash and chromogen.
A primary detection antibody is an antibody that binds only to the protein of concern. A secondary detection antibody is an enzyme-conjugated second antibody that binds to a primary antibody that has not been enzyme-conjugated. The addition of a substrate can produce a color. This is how detection is done.